ECE-1:一种与膜结合的金属蛋白酶,可催化大内甲蛋白-1的蛋白解活性
1Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235-9050.
Cell
|August 12, 1994
概括
研究人员确定了内甲林转化酶-1 (ECE-1),一种新型金属蛋白酶,对于产生血管活性内甲林-1 (ET-1) 至关重要. ECE-1为规范ET-1水平提供了一个潜在的目标.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 血管生物学 血管生物学
背景情况:
- 末端蛋白-1 (ET-1) 是一种强大的血管活性,在血管内皮细胞中产生.
- ET-1是由一个不活跃的前体,大内甲蛋白-1 (big endothelin-1) 通过一种特定的酶分裂合成的.
- 负责这种转换的酶,内甲蛋白转化酶 (ECE),代表了一个关键的监管点.
研究的目的:
- 为了识别和描述负责将大内素-1转化为成熟内素-1.1的酶.
- 为了研究已识别的酶的细胞表达和功能性质.
主要方法:
- 在培养细胞中克隆和转化内甲素转化酶-1 (ECE-1) cDNA.
- 评估大ET-1在转染细胞中的处理到成熟ET-1的过程.
- 描述了ECE-1作为一种与膜结合的中性金属蛋白酶.
主要成果:
- 鉴定ECE-1,一种新的膜结合的中性金属蛋白酶.
- 在体内内,CE-1在内皮细胞中表达丰富.
- 转染ECE-1cDNA使细胞能够分泌成熟的ET-1,处理内源性和细胞表面结合的大ET-1.
结论:
- ECE-1 是主要负责将大ET-1 转化为活性ET-1 的酶.
- ECE-1的本地化和功能表明它是ET-1生产的关键调节者.
- 在各种生理和病理条件下,ECE-1代表了调节ET-1活动的潜在治疗标.
相关概念视频
01:15Activation of Integrins
4.4K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
4.4K
01:33Enzyme-Linked Immunosorbent Assay
14.2K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
14.2K