一种新型的环素与MO15/CDK7结合,形成CDK激活酶
1Department of Physiology, University of California, San Francisco 94143-0444.
Cell
|August 26, 1994
概括
哺乳动物的CDK激活激酶 (CAK) 由MO15和环素H组成. 这种CDK7-环素H复合体激活了细胞周期进展所必需的其他环素依赖激酶 (CDK).
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 循环素依赖激酶 (CDK) 调节细胞循环.
- 激活CDKs需要通过CDK激活激酶 (CAK) 进行酸化.
- 哺乳动物CAK的组成以前没有被描述.
研究的目的:
- 为了净化和描述哺乳动物的CAK酶.
- 确定包括哺乳动物CAK.的子单位.
- 为了确定纯化的CAK全酶的功能.
主要方法:
- 从哺乳动物细胞中净化CAK.
- 蛋白质测序和cDNA克隆以识别子单元.
- 在体外溶解和激酶试验.
主要成果:
- 作为两个子单元复合体 (42 kDa 和 37 kDa) 净化的CAK.
- 42kDa的子单元是MO15的同类;37kDa的子单元是新型环素H.
- 复制后的CAK是CDK7-环素H,它可以激活CDK2和CDC2-环素复合体.
- 在MO15/CDK7中保存的氨酸对CAK活性至关重要.
结论:
- 哺乳动物CAK是一种CDK-环素复合体,被确定为CDK7-环素H.
- CDK7-环素H酸化并激活其他CDK,在细胞循环控制中发挥关键作用.
- 这一发现澄清了CDK激活和细胞周期调节的分子机制.
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