一种类似HMG的蛋白质,可以将转录激活剂切换为抑制剂
N Lehming1, D Thanos, J M Brickman
1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Nature
|September 8, 1994
概括
果虫DSP1蛋白质将转录因子Dorsal和NF-kappa B从激活剂转化为抑制剂. 这种切换取决于负调节元件,突出显示HMG蛋白质.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 转录调节涉及可以激活或抑制基因表达的蛋白质.
- 在早期胚胎中,Drosophila蛋白Dorsal,一个Rel家族成员,激活twist并抑制zen.
- 对于HMG蛋白在调节转录活性中的作用尚不完全理解.
研究的目的:
- 为了识别和描述Drosophila HMG1蛋白质参与调节背脊功能.
- 研究HMG蛋白质影响基因激活和抑制的机制.
- 为了确定HMG蛋白是否可以在激活和抑制模式之间切换转录调节器.
主要方法:
- 鉴定和克隆了一种新型Drosophila HMG1蛋白,DSP1.
- 在基因表达试验中分析DSP1对Dorsal和NF-kappa B活性的影响.
- 在DSP1中介的镇压中涉及的负面调节元件 (NRE) 的识别和描述.
主要成果:
- 一种新的Drosophila HMG1蛋白DSP1被确定.
- DSP1将转录激活器Dorsal转化为抑制器.
- 这种压制取决于位于背部结合部位附近的负调节元件 (NRE).
结论:
- DSP1作为一个开关,将Dorsal和NF-kappa B从转录激活剂转化为抑制剂.
- 与负监管元件 (NRE) 的相互作用对于此切换至关重要.
- 类似HMG的蛋白质,如DSP1和HMG I(Y),在确定调节性蛋白质的转录结果方面发挥着关键作用.
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