相关实验视频
Updated: Aug 11, 2026

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Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Published on: October 22, 2012
类决定了抗原呈现细胞中的MHCII类分子的寿命
C A Nelson1, S J Petzold, E R Unanue
1Center for Immunology, Washington University School of Medicine, St Louis, Missouri 63110.
Nature
|September 15, 1994
概括
抗原呈现细胞 (APC) 不同地处理扩展,影响它们的持久性和免疫性. 这种选择影响T细胞对-MHC复合物的识别.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞内处理从蛋白质抗原产生许多,但只有少数被免疫学识别.
- 对于H-2k小鼠,蛋白溶酶 (HEL) 的免疫主导T细胞表位是三片段 (残留物46-61).
- 核心T细胞表位 (残留52-61) 对于与MHCII类分子I-Ak. 结合至关重要.
研究的目的:
- 为了比较抗原呈现细胞 (APC) 处理和呈现HEL 52-61的情况.
- 为了确定扩展是否在APC选择和呈现方面具有优势.
- 研究长度对-MHC复合物的稳定性和免疫性的影响.
主要方法:
- 抗原呈现细胞 (APC) 用蛋白溶酶 (HEL) 进行培养.
- 恢复了含有HEL 52-61核心序列的自然加工的片.
- 分析了具有不同氨基和碳酸末端延伸的,以确定APC的处理性和稳定性.
主要成果:
- APCs将HEL处理成一个包含52-61核心序列和扩展的嵌套.
- -MHC复合体在与APC的关联时间中表现出显著的变化.
- -MHC复合物的持久性差异与其免疫性强度相关.
结论:
- APCs对扩展的处理影响了-MHC复合物的稳定性和免疫性.
- 体长度和由此产生的复杂持久性是T细胞识别的关键因素.
- 这种差异性选择机制有助于免疫系统识别特定-MHC复合物的能力.
相关概念视频
Cell-mediated Immune Responses
Overview
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Antigen Presenting Cells
The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
Antigen Processing Pathways
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

