14-3-3蛋白与蛋白激酶Raf的结合以及对其激活的影响
E Freed1, M Symons, S G Macdonald
1Onyx Pharmaceuticals, Richmond, CA 94806-5206.
Science (New York, N.Y.)
|September 16, 1994
概括
14-3-3蛋白与Raf激酶相互作用,并且需要其激活. 这些蛋白质在Raf介导的信号传导途径中起着至关重要的作用.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 拉夫是信号转导通路中的关键蛋白质激酶.
- 了解Raf激活机制对于破译细胞信号至关重要.
研究的目的:
- 确定与Raf相互作用并参与其激活的蛋白质.
- 阐明14-3-3蛋白在Raf功能中的作用.
主要方法:
- 酵母两种混合选,以识别Raf相互作用蛋白质.
- 哺乳动物细胞中的共免疫沉,以确认蛋白质相互作用.
- 在酵母和哺乳动物细胞中Raf激活的分析.
主要成果:
- 两种14-3-3蛋白被确定与Raf相互作用.
- 14-3-3蛋白与Ras结合有区别,它与Raf调节和激酶域结合.
- 14-3-3蛋白在哺乳动物细胞中与Raf结合,并通过激活的Ras促进膜局部化.
- 14-3-3蛋白的表达激活了酵母细胞中的Raf,类似于Ras.
结论:
- 14-3-3蛋白质是拉夫酶活性的新型调节者.
- 这些蛋白质对于Raf介导的信号传导至关重要.
- 14-3-3蛋白代表了信号通路相关疾病的潜在治疗点.
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