描述GroES的功能重要移动领域的特征
S J Landry1, J Zeilstra-Ryalls, O Fayet
1University of Texas Southwestern Medical Center, Dallas 75235-9041.
Nature
|July 15, 1993
概括
GroES共同主持人有一个移动循环,这对于GroEL的功能至关重要. 当GroES与GroEL结合时,这个循环变得不动,这表明调节蛋白质折叠的关键机制.
科学领域:
- 分子生物学分子生物学
- 蛋白质折叠 蛋白质的折叠
- 生物化学 生物化学
背景情况:
- GroES对于分子陪伴者GroEL的充分功能至关重要.
- GroES通过GroEL调节ATP结合/水解和基质释放/折叠.
- GroES和GroEL之间的精确分子相互作用机制尚不清楚.
研究的目的:
- 为了阐明GroES-GroEL相互作用的分子细节.
- 调查GroES结构动态在GroEL功能中的作用.
- 在复杂形成过程中识别GroES的结合部位和形状.
主要方法:
- 对GroES多环的移动性和可访问性的分析.
- 将groES基因中的突变映射到特定区域.
- 使用合成GroES和GroEL的生物化学研究.
主要成果:
- 原生GroES表现出高度移动和可访问的多环.
- 这种循环在GroES/GroEL复合体形成时失去流动性和可访问性.
- 在这个移动循环中,groES中的突变聚集在一起,影响GroEL相互作用.
- GroES循环可以将GroEL结合在一个特定的位置,形成针头形状.
结论:
- GroES的移动循环对于其与GroEL的互动至关重要.
- 循环灵活性对于与多个GroEL子单元同时结合至关重要.
- 这种相互作用在异质上调节GroEL/基质亲和力和蛋白质折叠效率.
相关概念视频
01:46Eukaryotic Compartmentalizations
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
For example, lysosomes in the animal cells...
01:19Contact-dependent Signaling
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Gap Junctions
In animal cells, gap junctions are formed...
02:12Short-distance Transport of Resources
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
01:18Membrane Domains
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
01:27Glucose Transporters
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
01:19Membrane Asymmetry Regulating Transporters
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...