转录因子PHO4的酸化由一个环林-CDK复合体,PHO80-PHO85进行
A Kaffman1, I Herskowitz, R Tjian
1School of Medicine, Department of Biochemistry and Biophysics, University of California at San Francisco 94143.
概括
研究人员发现了一种循环素依赖的激酶复合体,PHO80-PHO85,通过酸化转录因子PHO4来调节酵母PHO5基因转录. 这一发现揭示了循环-cdk复合体在细胞循环控制之外的新角色.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 基因规则 基因规则
背景情况:
- 酵母PHO5基因的转录被PHO2和PHO4转录因子激活.
- 在高酸盐条件下,PHO5转录被PHO80和PHO85抑制,这被认为会使PHO2和PHO4失活.
研究的目的:
- 研究PHO80和PHO85对PHO5转录的负调节机制.
- 描述PHO80和PHO85之间的相互作用以及它们在PHO4调节中的作用.
主要方法:
- 对PHO80.80进行同质分析.
- 在PHO80和PHO85.5之间进行了蛋白质与蛋白质相互作用研究.
- 通过PHO80-PHO85复合体对PHO4进行体外酸化试验.
主要成果:
- PHO80表现出对酵母环林的同质性.
- PHO80与PHO85相互作用,PHO85是一种与p34cdc2/CDC28.28相关的蛋白激酶.
- 复合物PHO80-PHO85复合物酸化了PHO4. 这种复合物是PHO80-PHO85复合物.
- PHO4酸化与PHO5转录的负调节相关.
结论:
- 一种新型的循环素依赖激酶 (CDK) 复合体,PHO80-PHO85,调节细胞循环控制之外的基因转录.
- PHO4 是 PHO80-PHO85 CDK 复合物的生理相关基质.
- 这项研究揭示了酵母中PHO5基因表达的新调节途径.
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