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Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
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人类干扰素马受体激活所需的辅助因子的识别和序列
J Soh1, R J Donnelly, S Kotenko
1Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635.
Cell
|March 11, 1994
概括
人类染色体21为人类干扰素 (Hu-IFN-gamma) 活性提供了必不可少的辅助因子,包括抗病毒保护. 这些因素,以及染色体6上的受体,对于对Hu-IFN-gamma的完整生物反应至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
- 病毒学 病毒学
背景情况:
- 人类干扰素 (Hu-IFN-) 调解关键免疫反应,包括I类HLA抗原诱导和抗病毒保护.
- 对Hu-IFN-gamma的敏感性需要在人类染色体6和21上编码的遗传信息.
研究的目的:
- 识别和表征由人类染色体21编码的辅助因素,这些因素有助于Hu-IFN-gamma生物活性.
- 阐明染色体21编码因子在Hu-IFN-玛受体信号传递和抗病毒防御中的特定作用.
主要方法:
- 利用基因组克隆探针识别表达特定物种附加因素的cDNA克隆.
- 评估了已识别的cDNA克隆重构成Hu-IFN-玛介导诱导I类HLA抗原的能力.
- 评估了这些cDNA克隆对抗脑肌心炎病毒 (EMCV) 抗病毒保护的影响.
主要成果:
- 识别了编码辅助因子的cDNA克隆,该辅助因子可以替代人类21号染色体,用于重组I类HLA抗原诱导.
- 证明,仅仅这一因素并不能对EMCV提供完全的抗病毒保护,这表明需要额外的染色体21编码的因素.
- 证实了人类21号染色体为不同的Hu-IFN-马函数编码了多个辅助因子.
结论:
- 已识别的辅助因子是介导Hu-IFN-的不同作用的因子家族的一部分.
- 人类21号染色体在Hu-IFN-马信号传递中起着复杂的作用,编码了与受体相关的因素和对完整的抗病毒活性至关重要的因素.
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