植物DNA病毒的两个蛋白质协调核和等离子体运输
A O Noueiry1, W J Lucas, R L Gilbertson
1Department of Plant Pathology, University of California, Davis 95616.
Cell
|March 11, 1994
概括
豆矮人马赛克 双子病毒运动蛋白 BR1 和 BL1 促进病毒DNA运输. BR1调解核出口,而BL1通过等离子体增强细胞对细胞的移动.
科学领域:
- 植物病毒学 植物病毒学
- 分子植物病理学 分子植物病理学
- 细胞生物学 细胞生物学
背景情况:
- 植物病毒通过等离子体系统传播.
- 病毒细胞间运动的机制尚未完全理解.
研究的目的:
- 研究豆矮体马赛克双子病毒 (单链DNA病毒) 的两个运动相关蛋白质 (BR1和BL1) 在病毒传输中的作用.
- 阐明蛋白质在核出口和细胞间移动中的功能.
主要方法:
- 对表达的病毒蛋白的体内功能分析.
- 在大肠杆菌中的蛋白质表达.
- 在植物细胞中进行微注射.
主要成果:
- BL1蛋白促进了广泛的细胞间移动.
- BL1增加了等离子体大小排除极限在中粒细胞中.
- BL1增强了双链DNA的细胞间移动.
- BR1蛋白调解了单链和双链病毒DNA从核中移动出来的过程.
结论:
- BR1和BL1蛋白质在病毒DNA传输中发挥着不同的但协调的作用.
- BR1和BL1促进病毒DNA穿越核和等离子体屏障的运动.
- 提供了植物细胞间宏分子运输的直接证据.
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