环素E控制S阶段的进展,其在Drosophila胚胎发生过程中的下调是阻止细胞增殖的必要条件
J A Knoblich1, K Sauer, L Jones
1Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tübingen, Federal Republic of Germany.
Cell
|April 8, 1994
概括
多菌的环素E (DmcycE) 控制了细胞循环通过S阶段的进展. 它的下调限制了胚胎的增殖,而它的子宫外表达可以重新启动细胞分裂.
科学领域:
- 发展生物学 发展生物学
- 细胞循环规则 细胞循环规则
- 分子遗传学 分子遗传学
背景情况:
- 大多数Drosophila胚胎的背上表皮细胞在分化16后停止分裂.
- 这种细胞循环的退出与多索菲拉环林E (DmcycE) 的下调有关.
研究的目的:
- 研究DmcycE在调节Drosophila胚胎生成期间细胞周期进展和增殖中的作用.
- 确定DmcycE对于S阶段进展的必要性及其对内核复制的影响.
主要方法:
- 通过子宫外表达研究分析DmcycE功能.
- 对DmcycE突变胚胎的检查,以评估其在S阶段和内复制中的作用.
主要成果:
- 宫外DmcycE表达在最终线粒分裂后诱导S阶段进入和额外的细胞周期.
- DmcycE对于S阶段的进展至关重要;DmcycE突变胚胎未能进行内复制.
- DmcycE与Dmcdc2c激酶形成一个复合体,以控制S相.
结论:
- 多虫环林E (DmcycE) 是S阶段进展的关键调节剂.
- 下调DmcycE对于限制胚胎细胞增殖至关重要.
相关概念视频
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
01:23Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:34Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
01:27Molecular Factors Affecting Cell Division
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...