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相关概念视频

Defenses Against Pathogens and Herbivores02:26

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
Overview of Cell Death01:30

Overview of Cell Death

Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Cellular Injury IlI: Cellular Death01:11

Cellular Injury IlI: Cellular Death

Cell death is the irreversible loss of cellular structure and function, representing the final stage of severe injury. It plays a key role in both normal physiology and disease.Types of Cell DeathThe two main types are necrosis and apoptosis, though others like necroptosis and pyroptosis also exist.Necrosis:Necrosis is an unregulated form of cell death caused by severe injury such as trauma, toxins, or ischemia. It is characterized by cell swelling, membrane loss, rupture, and leakage of...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...

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相关实验视频

Updated: Jul 22, 2026

Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
08:45

Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

Published on: September 9, 2009

植物的编程细胞死亡:一种由病原体触发的反应,与多个防御功能协调激活.

J T Greenberg1, A Guo, D F Klessig

  • 1Department of Genetics, Harvard Medical School, Boston, Massachusetts.

Cell
|May 20, 1994
PubMed
概括

突变的Arabidopsis thaliana植物缺乏加速细胞死亡2 (ACD2) 基因,自发地产生过敏反应 (HR). 这表明ACD2通常会抑制植物的细胞死亡.

科学领域:

  • 植物病理学 植物病理学
  • 分子遗传学 分子遗传学
  • 细胞生物学 细胞生物学

背景情况:

  • 过敏反应 (HR) 是一种涉及快速细胞死亡的植物防御机制.
  • HR通常是由病原体感染引发的,并且被认为涉及编程细胞死亡途径.

研究的目的:

  • 调查加速细胞死亡2 (ACD2) 基因在调节植物编程细胞死亡中的作用.
  • 在acd2突变植物中观察到的自发HR类表型的特征.

主要方法:

  • 对Arabidopsis thaliana突变的遗传分析.
  • 在acd2植物中自发性病变的表型特征.
  • 对与防御相关的基因表达和与HR相关的分子标记物的分析.

主要成果:

  • 突变的Arabidopsis thaliana植物在ACD2基因中出现病变,在没有暴露于病原体的情况下表现出自发的HR类症状.
  • 这些自发性病变表现出HR的典型特征,包括细胞壁的修饰,病原体的耐药性和酸和抗菌化合物的积累.
  • 与防御相关的基因转录也在受影响的组织中升高.

结论:

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Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
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Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

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Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
08:45

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Published on: September 9, 2009

A β-glucuronidase (GUS) Based Cell Death Assay
07:35

A β-glucuronidase (GUS) Based Cell Death Assay

Published on: May 6, 2011

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

  • 在植物中,ACD2基因在负面调节编程细胞死亡途径方面发挥着至关重要的作用.
  • 失去了ACD2功能导致过敏反应自发激活,表明其功能抑制HR.
  • ACD2是植物细胞死亡和防御的遗传控制的关键组成部分.