印记和X染色体计数机制决定了小鼠早期发育中的Xist表达
G F Kay1, S C Barton, M A Surani
1Section of Comparative Biology, Medical Research Council Clinical Research Centre, Harrow, England.
Cell
|June 3, 1994
概括
父母印记最初控制小鼠胚胎中的Xist基因表达,导致父系Xist等位基因激活. 这种印记后来被删除,允许X染色体计数来调节Xist表达.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
- 发展生物学 发展生物学
背景情况:
- 雌性哺乳动物的X无活化使一个X染色体沉默,以使基因剂量相等.
- 基因组织 (X-无活性的特异性转录) 对于启动X的无活化至关重要.
- 在小鼠中,早期的Xist表达被打印出来,有利于父亲的等位基因.
研究的目的:
- 调查父母印记在早期Xist表达中的作用.
- 了解从印记到随机Xist表达的过渡.
- 为了确定Xist表达的潜在调节者.
主要方法:
- 使用单亲老鼠胚胎来隔离父母印记效应.
- 分析Xist等位基因特定的表达模式.
- 观察X染色体计数机制.
主要成果:
- 基因表达最初仅由父母的印记决定,其中父亲的等位基因表达,母亲的等位基因被抑制.
- 这种印记在压缩的莫鲁拉阶段被删除.
- 然后,X染色体数决定了适当的Xist表达.
- 一个新的母体表达印记基因可能调节Xist.
结论:
- 父母印记是小鼠胚胎初始Xist表达的主要驱动因素.
- 一个开关发生在X染色体计数覆盖Xist调节的印记的地方.
- 需要进一步的研究来阐明新建议的印记基因的作用.
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