相关实验视频
Updated: Jul 18, 2026

09:42
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
概括
来自小鼠髓瘤细胞系的体细胞混合体稳定地表达父母免疫球蛋白链. 然而,频繁的分离克隆出现,随着时间的推移失去特定重或轻链的合成.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
背景情况:
- 身体细胞杂交技术使得混合细胞系的产生成为可能.
- 鼠标髓瘤细胞系被用来产生杂交瘤.
- 杂交细胞中的免疫球蛋白链表达需要表征.
研究的目的:
- 隔离和描述不同小鼠髓瘤细胞系之间的体细胞杂交.
- 分析这些杂交体中免疫球蛋白重 (H) 和轻 (L) 链的表达.
- 评估免疫球蛋白表达的稳定性,并确定潜在的遗传不稳定性.
主要方法:
- 用修改的技术隔离体细胞杂交.
- 通过HAT或HAT-ouabain选择程序选择混合细胞.
- 通过染色体数分析和H-2表面抗原表达来确认杂交性质.
主要成果:
- 三种混合细胞系 (IgG2B, κ X IgG2a, κ; IgG1, κ X IgG2b, κ; IgG1, κ X IgM, λ) 已经成功生成.
- 混合细胞表达了H和L链的双亲类型,没有新的链形成.
- 在混合分子中观察到父母H和/或L链的二硫化物结合混合物.
- 虽然稳定长达13个月,但频繁分离的克隆出现,失去父链合成.
结论:
- 来自小鼠髓瘤细胞系的体细胞杂交,保持了父母免疫球蛋白链的表达.
- 混合细胞由于H和L链的二硫化物结合混合物而表现出分子异质性.
- 尽管最初的稳定性,导致免疫球蛋白链表达丧失的遗传不稳定性在亚克隆中经常发生.
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