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由淋巴细胞抗原CD38催化的循环ADP-ribose的形成和水解

M Howard¹, J C Grimaldi, J F Bazan

¹DNAX Research Institute, Palo Alto, CA 94304.

Science (New York, N.Y.)

|November 12, 1993

概括

CD38是淋巴细胞上的一种酶,从NAD+中产生循环ADP-ribose (cADPR). 这种分子增强了B细胞的增殖,这表明CD38是CD38.

科学领域:

免疫学免疫学免疫学
生物化学生物化学
分子生物学分子生物学

背景情况:

CD38是一种在B和T淋巴细胞上发现的糖蛋白.
CD38与代谢尼古丁胺氨酸二核酸 (NAD+) 的酶具有结构上的相似之处.

研究的目的:

为了研究CD38.8的酶活性.
通过其酶产物探索CD38在淋巴细胞功能中的作用.

主要方法:

为小鼠CD38.8的细胞外域构建和表达补充DNA.
净化复合溶性CD38到同质性.
用NAD+作为基质测试可溶性CD38的酶活性.
将净化循环ADP-ribose (cADPR) 给激活的小鼠B细胞.

主要成果:

可溶性CD38显示,它具有从NAD+中催化循环ADP-ribose (cADPR) 的形成和水解的能力.
纯化cADPR被发现可以增强激活的小鼠B细胞的增殖反应.

结论:

CD38的酶活性,特别是其对cADPR的产生,可能在调节淋巴细胞增殖方面发挥重要作用.
CD38在淋巴细胞中的功能可能与其调动代谢物cADPR.相关.

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