相关实验视频
Updated: Sep 13, 2026
09:42
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
产生一种产生免疫球蛋白卡帕链的小鼠菌株,具有人类常数区域
1Institute for Genetics, University of Cologne, Germany.
Science (New York, N.Y.)
|November 19, 1993
概括
研究人员创建了一个生产人性化抗体的小鼠模型. 这一进步使得使用自然免疫过程生成高亲和度人性化抗体成为可能,为免疫治疗提供了一个有前途的选择.
科学领域:
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
- 基因工程是一种基因工程.
背景情况:
- 与动物抗体相比,人性化抗体提供了优越的免疫治疗潜力.
- 开发有效的人性化抗体对于先进的治疗方法至关重要.
研究的目的:
- 为了创建一个小鼠模型来产生高亲和度的人性化抗体.
- 为了利用基因向来替换小鼠卡帕轻链基因与人类对应物.
主要方法:
- 用基因向来替换小鼠卡帕光链常量 (C) 区域基因,用人类的C卡帕基因.
- 对C kappa R突变同卵性小鼠进行了抗体产生和免疫反应的分析.
主要成果:
- 小鼠产生正常的抗体度与虚构的卡帕光链.
- 在这些小鼠中观察到对哈蛋白结合物的正常免疫反应.
结论:
- 开发的小鼠菌株是生产高亲和度人性化抗体的可行工具.
- 这项技术利用体质突变和选择产生抗体.
相关概念视频
02:29In-vitro Mutagenesis (Gene Knockouts)
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
01:31Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
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