Cdi1,一个人类G1和S相蛋白酸酶,与Cdk2结合
J Gyuris1, E Golemis, H Chertkov
1Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Cell
|November 19, 1993
概括
我们发现了循环素依赖性激酶相互作用体1 (Cdi1),一种与循环素依赖性激酶相互作用的新型蛋白质酸酶. Cdi1 一个心脏.
科学领域:
- 分子生物学分子生物学
- 细胞循环规则 细胞循环规则
- 酶学 是一种酶学.
背景情况:
- 循环素依赖激酶 (CDK) 是细胞循环的关键调节者.
- 了解CDK相互作用器是解读细胞循环控制机制的关键.
研究的目的:
- 为了识别与循环林依赖激酶相互作用的新型人类蛋白质.
- 描述新发现的CDK交互因子的功能和酶活性.
主要方法:
- 利用酵母相互作用陷,一种遗传选择系统,以隔离相互作用的蛋白质.
- 进行生物化学测试以确定酸酶活性和基质特异性.
- 研究了人类 (HeLa) 细胞中蛋白质复合体的形成.
- 在酵母和HeLa细胞中进行了细胞周期进展研究.
主要成果:
- 使用交互陷识别了人类循环素依赖激酶交互因子1 (Cdi1).
- 在酵母中,Cdi1与几种人类的CDKs (Cdc2,Cdk2,Cdk3) 相互作用.
- 在实验室中,Cdi1表现出氨酸酸酶活性,由一种催化无活性的突变体证实.
- 在HeLa细胞中,Cdi1与Cdk2形成稳定的复合体,并在G1到S过渡过程中得到表达.
- 过度表达Cdi1延迟了酵母和HeLa细胞的细胞周期进展,这取决于其酸酶活性.
结论:
- Cdi1是一种新型的蛋白质酸酶,与循环林依赖性激酶复合.
- Cdi1的酸酶活性在调节细胞周期进展方面发挥作用.
- 这一发现为复杂的细胞循环控制网络提供了新的见解.
相关概念视频
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Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
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The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...