局部的刺活动控制了Drosophila胚胎中无翼转录的空间限制
1Molecular Embryology Laboratory, ICRF Development Biology Unit, Department of Zoology, Oxford, UK.
Nature
|December 9, 1993
概括
在Drosophila胚胎中无处不在的刺 (hh) 信号在特定细胞中异位激活无翼 (wg). 细胞激活wg的能力与信号接收是分开的,需要融合基因产物.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 果胚胎中的细胞模式依赖于细分极性基因.
- 无翼 (wg) 和刺 (hh) 是编码分泌信号蛋白的关键分段极性基因.
- wg转录是hh活动的目标,建议空间控制取决于hh信号范围和细胞能力.
研究的目的:
- 测试该模型,wg表达的空间控制取决于hh信号范围和细胞能力.
- 研究Drosophila胚胎发育中的hh信号传导机制.
主要方法:
- hedgehog (hh) 基因的无处不在表达是使用 Hsp70 促销器驱动的.
- 对无翼 (wg) 基因激活进行了监测.
- 另一个hh活动的目标被用来评估细胞能力.
- 研究了融合基因在WG激活中的作用.
主要成果:
- 无处不在的hh表达导致了每个类细分体内的细胞子集的异位WG激活.
- 发现细胞表达wg的能力与它们接收hh信号的能力是独立的.
- wg激活被证明需要部分极性基因的功能融合.
结论:
- 通过hh激活wg的空间限制不仅仅是由于有限的hh信号范围或差异细胞能力.
- 细胞对WG表达的能力与信号接收不同.
- 合并的基因产物,一种氨酸/氨酸激酶,可能会转导hh信号.
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