一种小型GTP依赖的调节性蛋白质ADP-ribosylation因子,刺激了脂酶D活性
H A Brown1, S Gutowski, C R Moomaw
1Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9041.
Cell
|December 17, 1993
概括
脂酶D (PLD) 的活性由一种细胞系因子增强,称为ADP-Ribosylation Factor (ARF). 这种ARF蛋白对PLD至关重要.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 脂酶D (PLD) 水解酸丁胆,产生酸和胆.
- PLD活动受到各种细胞因子的调节,包括细胞质成分.
- ADP-Ribosylation Factors (ARFs) 是已知的细胞内囊泡流通的调节者.
研究的目的:
- 开发一种测试方法来测量细胞制剂中的PLD活性.
- 识别和描述增强PLD活动的细胞系因子.
- 研究ARF蛋白在PLD调控中的作用.
主要方法:
- 开发一种基于外源基质的测定方法来检测PLD活性.
- 从牛脑细胞醇中净化细胞系因子.
- 使用生化和分子技术对纯化因子进行表征.
- 重组基化ARF1.1的表达和测试.
主要成果:
- 鉴定出了一种细胞分裂因子,可显著增强膜中的PLD活性.
- 这种因子经过净化,被确定为ADP-Ribosylation Factor (ARF) 子家族的成员.
- 与非基化形式相比,重组基化ARF1显示了PLD的增强激活.
- ARF蛋白对于GTPγS依赖的PLD刺激至关重要.
结论:
- ARF蛋白质在调节脂酶D活性方面发挥着突出作用.
- 通过PLD,ARF可以作为第二信使生成的关键组件.
- 这一发现将ARF介导的囊泡贩运与涉及PLD的信号转导通路联系起来.
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