病毒病毒基因组末端区域的潜在毒性决定因素
R F Massung1, J J Esposito, L I Liu
1Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Nature
|December 23, 1993
概括
确定了大型麻疹病毒 (VAR-BSH) 的完整基因组序列,揭示了187种蛋白质. 大多数蛋白质与疫苗病毒相似,但37种蛋白质显示出型病毒特异性,有助于致病性.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 根除天花在抗菌药物运动中取得了重大成功.
- 了解大 Variola 病毒基因组对于研究天花至关重要.
- 之前的研究已经对疫苗病毒进行了测序,这是一种相关的毒素病毒.
研究的目的:
- 为了确定大 Variola 病毒 (VAR-BSH) 基因组的完整核酸序列.
- 识别可能涉及致病性和宿主范围的病毒蛋白质.
- 为了比较疹病毒基因组与疫苗病毒的基因组.
主要方法:
- 全基因组测序大麻疹病毒,孟加拉国-1975 (VAR-BSH) 菌株.
- 生物信息分析用于识别开放的读取框架和预测蛋白质序列.
- 进行比较基因组学,以分析与哥本哈根疫苗病毒菌株 (VAC-CPN) 的相似之处和差异.
主要成果:
- 确定了VAR-BSH的完整基因组序列 (186,102个基对).
- 在病毒基因组中发现了187种假定蛋白质.
- 150种蛋白质与疫苗病毒蛋白质具有很高的相似性.
- 37种蛋白质表现出小 Variola 特定的序列或显著的分歧.
结论:
- 基因组测序为脊髓灰质炎病毒研究提供了全面的资源.
- 脊髓灰质炎特异性蛋白质可能在病毒的致病性和宿主热带性中发挥关键作用.
- 对比分析强调了与疫苗病毒相比,导致疹病毒毒性的遗传差异.
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