起源识别复合体 (ORC) 在S. cerevisiae中的转录沉默和DNA复制中
M Foss1, F J McNally, P Laurenson
1Department of Molecular and Cellular Biology, University of California, Berkeley 94720.
Science (New York, N.Y.)
|December 17, 1993
概括
原始识别复合体 (ORC) 在酵母DNA复制和转录沉默中起着双重作用. 在ORC2中的突变破坏了这两种过程,将复制起源与基因沉默机制联系起来.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在Saccharomyces cerevisiae中的HMR-E沉声器通过阻止蛋白质-DNA相互作用来调节基因表达.
- 沉默器功能与在特定来源启动DNA复制有关.
研究的目的:
- 为了确定在HMR位点参与转录沉默的基因.
- 阐明已识别的基因在DNA复制和沉默中的作用.
主要方法:
- 查影响HMR基因表达的突变.
- 标志着鉴定基因 (ORC2) 中温度敏感突变的特征.
- 评估细胞周期进展和等离子体稳定性.
主要成果:
- 确定了一种基本的基因ORC2,它编码了起源识别综合体 (ORC) 的一个组成部分.
- 在允许的温度下,ORC2 (orc2-1) 的突变破坏了转录沉默.
- 在限制性温度下,ORC2-1导致细胞循环停止,并增加了等离子体损失,表明复制缺陷.
结论:
- 在体内,ORC对于染色体DNA复制和转录沉默都至关重要.
- 在DNA复制启动和表观遗传沉默机制之间存在功能联系.
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