赛马福林基因编码了一个跨膜和分泌的生长引导分子家族
A L Kolodkin1, D J Matthes, C S Goodman
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Cell
|December 31, 1993
概括
这项研究识别和描述了虫和人类中的semaphorin基因,揭示了它们在神经发育和成年人生存中的关键作用. 赛马福林I指导在上皮细胞边界的感觉生长.
科学领域:
- 发育生物学是发展生物学.
- 神经科学是一个神经科学.
- 分子遗传学 分子遗传学
背景情况:
- 半素I (Sema I) 引导虫肢体芽中的表皮边界的感觉生长.
- 赛马福林是一种保存的蛋白质家族,涉及到细胞指导.
研究的目的:
- 克隆和表征Drosophila和人类中的semaphorin基因.
- 为了研究在神经发育和行为中semaphorin基因的功能.
主要方法:
- 基因克隆和表征drosophila和人类的semaphorin基因.
- 识别相关的病毒塞马福林序列.
- 在神经元和肌肉中对Drosophilasemaphorin基因的表达分析.
- 在Drosophila中进行基因分析以确定基因基本性.
主要成果:
- 克隆和特征化了两种Drosophila半蛋白基因 (D-Sema I和D-Sema II) 和一个人类半蛋白基因 (Sema III).
- D-Sema I是一种跨膜蛋白,而D-Sema II和人类Sema III是分泌的蛋白质.
- D-Sema I和D-Sema II以神经元和肌肉的子集来表达.
- 德洛索菲拉塞马II对于成年人的行为和生存至关重要.
结论:
- 赛马福林在各个物种中都存在,在神经发育中起着关键作用.
- 德洛索菲拉 (Drosophila semaphorin II) 对于正常的神经系统功能至关重要,影响行为和生存.
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