绿色光蛋白作为基因表达的标记物

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Updated: Nov 8, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

M Chalfie1, Y Tu, G Euskirchen

  • 1Department of Biological Sciences, Columbia University, New York, NY 10027.

Science (New York, N.Y.)
|February 11, 1994
PubMed
概括

来自Aequorea victoria的绿色光蛋白 (GFP) 可以在细菌和虫中表达,在没有外部化学物质的情况下产生光. 这使得GFP能够实时跟踪活细胞内的基因活动和蛋白质位置.

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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...