具有不同对ecdysone的变形反应的虫组织表达不同的ecdysone受体异型
W S Talbot1, E A Swyryd, D S Hogness
1Department of Biochemistry, Stanford University School of Medicine, California 94305.
Cell
|July 2, 1993
概括
果中的ecdysone受体基因产生了三种异型 (EcR-A,EcR-B1,EcR-B2). 这些异构体在组织中的不同组合驱动了复杂的转化过程,由激素ecdysone触发.
科学领域:
- 发展生物学 发展生物学
- 内分泌学 在内分泌学.
- 遗传学 是一个遗传学.
背景情况:
- 在 *Drosophila melanogaster* 中的转变是由类固醇激素ecdysone 启动的.
- 埃克迪松诱导成体结构从图像组织的发展和幼虫组织的退化.
- 埃克迪松引发各种组织特异性反应的精确分子机制尚未完全理解.
研究的目的:
- 为了研究ecdysone受体异型在调解Drosophila*转型过程中的多种细胞反应中的作用.
- 确定不同ecdysone受体异型及其mRNA在发育过程中的表达模式和时间分布.
主要方法:
- 利用异型特异性单克隆抗体来检测和定位D. melanogaster*组织中的ecdysone受体 (EcR) 异型.
- 在整个发育过程中分析了EcR异型及其相应的mRNAs的时间表达特征.
- 相关的单形表达模式与特定的发育阶段,以ecdysone脉冲标记.
主要成果:
- *EcR*基因编码了三个不同的异型:EcR-A,EcR-B1和EcR-B2,共享共同的DNA和激素结合域,但在它们的N端区域上有所不同.
- 不同的EcR异型组合在转化开始时表达在不同的ecdysone点组织中.
- 时间表达特征显示,特定的EcR异型在与ecdysone脉冲相关的不同发育阶段占主导地位.
结论:
- 特定组织中EcR异型的差异表达是观察到的各种变形反应的基础.
- EcR异形的组合作用提供了一个精确调节复杂发育过程的机制,如变形.
- 了解EcR异形函数对于破译发展的荷尔蒙控制至关重要.
相关概念视频
02:32Position-effect Variegation
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
02:45The Ratio of X Chromosome to Autosomes
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...