在HLA-DR结合中,有杂乱性和异位基因特异性
J Hammer1, P Valsasnini, K Tolba
1Roche Milano Ricerche, Italy.
Cell
|July 16, 1993
概括
人类MHCII类多态性影响结合. 我们在-MHC基因中确定了特定的结残留物,揭示了DR分子的共同和异位基因特定的结合特征.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 主性基因相容性复合体 (MHC) II 类分子对于免疫反应至关重要.
- 这些葡萄糖蛋白具有高度多态性,并为CD4+ T细胞提供片段.
- 了解多态性对-MHC相互作用的影响是关键.
研究的目的:
- 研究人类MHCII类多态性对结的影响.
- 通过特定的DR等位基因来定义认知的序列动机.
主要方法:
- 利用M13菌体显示库进行类选择.
- 隔离的菌体与DRB1*0401和DRB1*1101的MHCII类分子结合.
- 对选定的菌体进行了序列分析,以确定结合基因.
主要成果:
- 鉴定了与DR分子结合的酸中的特定位置的 anchor 残留物.
- 定义了三个不同的DR绑定动机.
- 在位置1和4发现了共同的基残留物,在位置6发现了等位基因特异性残留物.
结论:
- 已确立的MHCII类结乱性和特异性的生物物理基础.
- 已证明的基因基因特异性基残留物有助于选择性的识别.
- 提供了对T细胞识别分子机制的见解.
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