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Myo-mechanical Analysis of Isolated Skeletal Muscle

Myo-mechanical Analysis of Isolated Skeletal Muscle

Published on: February 23, 2011

缺少肌肉肌激酶的小鼠的骨肌肉缺乏爆发活动

J van Deursen¹, A Heerschap, F Oerlemans

¹Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands.

|August 27, 1993

概括

缺乏肌肉肌酸激酶 (CK) 基因的小鼠无法执行爆发活动,尽管能量水平正常. 这表明活跃肌肉中能量利用存在替代途径.

科学领域:

肌肉生理学肌肉生理学
生物能源学生物能源学
生物化学生物化学

背景情况:

肌肉中快速能量缓冲过程中,肌肉中的肌酸激酶-酸肌酸 (CK-PCr) 系统至关重要.
了解其精确的生理作用需要调查功能受损的条件.

研究的目的:

阐明CK-PCr系统在肌肉生物能量学中的生理意义.
为了研究肌肉肌酸激酶 (M-CK) 基因中无基因突变的功能后果.

主要方法:

生殖线M-CK基因零突变小鼠的世代.
评估肌肉力量,收缩性质和纤维类型特征.
分析高能酸盐水平 (PCr,ATP) 和休息和运动期间的能量交换率.

主要成果:

缺乏M-CK的小鼠表现出正常的绝对肌肉力量,但损害了突发活动性能.
快速动的肌肉纤维显示线粒体体积增加,以及增强的糖核分解/糖核分解能力.
虽然静止PCr和ATP水平正常,但PCr-ATP交换率显著降低 (至少20倍).
运动期间PCr水平下降,表明M-CK独立的利用途径.

结论:

在强烈的肌肉活动中,M-CK酶对于高能量的酸盐有效转移至关重要.
肌肉生物能学可以通过增加线粒体和糖分分解能力来适应M-CK缺乏.
其他途径有助于在运动肌肉中利用脂蛋白,独立于M-CK介导的缓冲.

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