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In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
Published on: July 28, 2010
没有CD4的辅助T细胞:控制CD4缺乏的小鼠中的莱什曼病
R M Locksley1, S L Reiner, F Hatam
1Department of Medicine, University of California, San Francisco 94143-0654.
Science (New York, N.Y.)
|September 10, 1993
概括
功能性辅助T细胞可以在没有CD4的情况下发展. 即使没有CD4的CD8T细胞,也可以通过产生干扰素-,通过产生干扰素-来调解寄生虫耐药性,这挑战了对T细胞发育的传统观点.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- T淋巴细胞生物学T淋巴细胞生物学
背景情况:
- CD4和CD8糖蛋白定义了不同的T淋巴细胞系.
- 一个CD4基因突变损害了辅助T细胞的发育.
- CD4在T细胞系承诺和功能中的作用已经得到了很好的证实.
研究的目的:
- 在没有CD4的情况下研究辅助T细胞的发育和功能.
- 为了确定辅助T细胞功能是否存在替代途径.
主要方法:
- 使用CD4-null小鼠进行实验感染Leishmania.
- 分析的T细胞种群,包括CD8+αβT细胞受体+T细胞.
- 通过主要基因相容性复合体等级II进行评估的T细胞限制.
- 在寄生虫抗原挑战时测量了干扰素-玛产量.
主要成果:
- 在感染莱什马尼亚病毒的CD4-null小鼠中确定了一种功能性辅助T细胞群.
- 这些细胞被描述为CD8-αββT细胞受体+T细胞.
- 对于这些T细胞,已证明主要的组织相容性复杂II类限制.
- 在对寄生虫抗原的反应中确认了干扰素-玛的产生.
结论:
- T淋巴细胞谱系的承诺和外围功能并不完全取决于CD4.
- CD8 T细胞可以表现出辅助功能,包括细胞因子的生产,独立于CD4.
- 这一发现扩大了对T细胞可塑性和免疫反应机制的理解.
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