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在整个培养的小鼠胚胎中,血管活性肠道的生长因子功能
P Gressens1, J M Hill, I Gozes
1Laboratory of Experimental Neuropathology, National Institute of Neurological Disorders and Stroke, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Nature
|March 11, 1993
概括
血管活性肠 (VIP) 作为胚胎早期发育的生长因子,促进中枢神经系统 (CNS) 的生长和细胞增殖. 一种VIP对抗剂抑制了这些促进生长的效果,突出显示了VIP的作用.
科学领域:
- 发展生物学 发展生物学
- 神经科学是一个神经科学.
- 内分泌学 在内分泌学.
背景情况:
- 调节神经元形成后早期中枢神经系统 (CNS) 增长的因素在很大程度上是未知的.
- 血管活性肠 (VIP) 受体在胚胎神经系统中很普遍,对神经组织有已知的热带性和线粒性作用,但对某些瘤有抑制作用.
研究的目的:
- 调查VIP在胚胎在早期植入后发育过程中的生长中的作用.
- 确定VIP对中枢神经系统生长和细胞分裂的影响.
主要方法:
- 整个植入后胚胎培养被用于实验.
- 给出了血管活性肠 (VIP) 和一种VIP抗剂,以评估它们对胚胎生长参数的影响.
- 在试管体内使用自发射线图来表征VIP受体.
主要成果:
- VIP显著刺激了胚胎生长,经过4小时化后,胚胎体积,DNA和蛋白质含量以及S相细胞的增加,体现了胚胎细胞数量的增加.
- 一种VIP抗剂显著抑制了VIP介导的生长增长,并完全抑制了VIP刺激的中枢神经系统中的线粒分裂.
- 治疗VIP抗剂减少了38%的非神经组织中的线粒分裂,并揭示了GTP敏感和GTP不敏感的VIP受体的差异调节.
结论:
- 血管活性肠 (VIP) 在早期植入后胚胎中起到关键生长因子的作用.
- VIP通过多个VIP受体发挥其增长促进作用,显示出组织特异性反应.
- 这些发现阐明了VIP在调节胚胎中枢神经系统发育中的新角色.
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