在老鼠淋巴体中,由GnRH诱导的振荡刺激的节律性外细胞分裂
1Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195.
Science (New York, N.Y.)
|April 2, 1993
概括
激素释放激素 (GnRH) 触发 (Ca2+) 从垂体细胞中的细胞内储存释放,驱动激素分泌. 这项研究证实,GnRH刺激的分泌与这些细胞内Ca2+信号密切相关.
科学领域:
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
- 神经科学是一个神经科学.
背景情况:
- 下垂体淋巴体是调节生殖功能的关键细胞.
- 淋巴结核释放激素 (GnRH) 是淋巴结核功能的一个关键调节剂.
- 信号传递在细胞过程中起着至关重要的作用,包括荷尔蒙分泌.
研究的目的:
- 为了研究细胞内 (Ca2+) 释放在GnRH刺激的淋巴激素分泌中的作用.
- 确定单个淋巴细胞中细胞质Ca2+度 ([Ca2+]i) 振荡和外细胞形成之间的关系.
主要方法:
- 在单个已识别的淋巴细胞中同时测量细胞质自由Ca2+度 ([Ca2+]i) 和外细胞形成.
- 使用因诺醇1,4,5-三酸盐 (IP3) 敏感储存物释放Ca2+.
- 采用子IP3的光解来诱导Ca2+的升高,并评估细胞外化.
- 研究Ca2+缓冲和细胞外Ca2+缺失对分泌的影响.
主要成果:
- GnRH诱导了由 pituitary gonadotropes 中的 IP3 敏感储存中的节奏性 Ca2+ 释放.
- [Ca2+]i的升高与外细胞突发直接相关,表明密切的合.
- 细胞内Ca2+释放,而不是通过电压通的细胞外Ca2+流入,主要驱动GnRH刺激的细胞外.
- 子IP3的光解成功触发了外细胞分裂,证实了细胞内Ca2+释放的作用.
结论:
- 刺激性腺激素分泌与IP3敏感储存中的细胞内Ca2+释放密切相关.
- 在分泌细胞中因激素诱导的Ca2+振荡优化激素输出,同时防止持续高的Ca2+水平导致细胞毒性.
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