克塞诺普斯IP3受体:在卵细胞和卵子中的结构,功能和定位
Cell
|May 7, 1993
概括
伊诺三酸盐受体 (IP3R) 在Xenopus卵激活中起着关键作用. 这项研究确定了Xenopus IP3R (XIP3R),并揭示了它在受精期间 (Ca2+) 波形成和传播中的关键作用.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 卵子激活是繁殖中的关键过程,涉及受精开始的快速细胞变化.
- (Ca2+) 信号在触发卵子激活中起着核心作用.
- 伊诺西三酸盐受体 (IP3R) 是细胞内Ca2+释放的关键媒介.
研究的目的:
- 为了研究IP3受体 (IP3R) 在Xenopus蛋激活中的作用.
- 描述Xenopus IP3R (XIP3R) 及其在卵细胞成熟和受精期间的局部化.
主要方法:
- 隔离和分析编码Xenopus IP3R的cDNA克隆.
- 功能表达研究以确定IP3R域.
- 免疫细胞化学分析以可视化XIP3R在卵细胞和卵中的分布.
主要成果:
- 鱼IP3R (XIP3R) 具有IP3绑定域和Ca2+通道区域.
- XIP3R最初定位在胚胎内膜网状结构和未成熟卵细胞的周核区域.
- 在排卵和受精时,XIP3R重新分布到皮质区域,显示出动态变化.
- 在未受精卵的皮质区域中,XIP3R被密集丰富,并在受精后发生显著的再分配.
结论:
- 鸟IP3R (XIP3R) 是一个关键的组成部分,参与调节在卵激活期间的释放.
- 在XIP3R局部化中的动态变化表明它在Xenopus中受精期间在波的形成和传播中起着主要作用.
- 了解XIP3R功能,可以了解受精和早期胚胎发育的基本机制.
更多相关视频
相关概念视频
Fertilization
68.8K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
68.8K
Phosphoinositides and PIPs
7.7K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
7.7K
IP3/DAG Signaling Pathway
12.5K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.5K
Structure of Cadherins
4.0K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
4.0K
Catenins
2.2K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.2K
Immunoprecipitation
5.6K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
5.6K


