谷氨酸抗剂和细胞外酸性的神经保护作用
D A Kaku1, R G Giffard, D W Choi
1Department of Neurology and Neurological Sciences, Stanford University School of Medicine, CA 94305.
概括
细胞外酸性保护神经元免受缺氧,增强谷氨酸抗剂的神经保护作用. 这种组合提供了显著的神经元存活率,即使在长期低氧条件下.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 病理生理学 病理生理学
背景情况:
- 谷氨酸抗剂在体内和体外表现出神经保护作用,防止缺氧损伤.
- 之前的体外研究没有复制体内缺氧-缺血症特征的酸性条件.
研究的目的:
- 在模仿体内缺氧-缺血症的条件下研究细胞外酸度和谷氨酸抗剂的神经保护作用.
- 为了确定细胞外酸性是否增强了谷氨酸受体对抗剂在保护神经元中的有效性.
主要方法:
- 鼠类皮质培养物受到氧气和葡萄糖剥夺.
- 评估了单独和与N-甲基-D-酸盐 (NMDA) 和α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) - kainate对抗剂结合的细胞外酸性对神经元死亡的影响.
主要成果:
- 单独的细胞外酸性会在缺氧和缺血糖期间减少神经元死亡.
- 细胞外酸性和谷氨酸抗剂 (NMDA和AMPA-酸盐) 的组合显著降低了神经元死亡.
- 用组合方法治疗的神经元耐受长时间的氧气和葡萄糖剥夺,显示与星球细胞相比的生存率.
结论:
- 细胞外酸性提供神经保护,不仅仅是减弱电离子类谷氨酸受体激活.
- 细胞外酸性和谷氨酸抗剂的组合提供了优越的神经保护与单独谷氨酸抗剂相比.
- 这些发现表明,对于涉及缺氧-缺血症的疾病,这可能是一个潜在的治疗策略.
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