罗斯肉瘤病毒的A亚组的受体与低密度脂蛋白受体有关
P Bates1, J A Young, H E Varmus
1Department of Microbiology and Immunology, University of California, San Francisco 94143-0502.
Cell
|September 24, 1993
概括
细胞受体是逆转录病毒进入的关键. 研究人员确定了小型,低密度脂蛋白受体相关的蛋白质作为Rous瘤病毒亚组A的细胞受体,使其能够进入细胞.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 细胞受体对于逆转录病毒进入宿主细胞至关重要.
- 之前的研究已经确定了一种基因,该基因对罗斯肉瘤病毒亚组A (RSV(A)) 具有敏感性.
研究的目的:
- 为了识别和表征RSV中涉及的细胞受体.
- 为了阐明RSV的分子机制(A) 细胞入口.
主要方法:
- 隔离了敏感性基因的长同类基因.
- 对编码细胞受体的替代拼接加工基因的生成和分析.
- 在哺乳动物细胞中发现的基因的表达,以评估易感性.
- 使用抗体来阻止病毒感染.
主要成果:
- 确定了两种替代拼接的基因,这些基因编码RSV (A) 的细胞受体.
- 预测的受体蛋白质是小的,与共享的细胞外域相似的膜相关,类似于低密度脂蛋白受体 (LDLR).
- 这些基因的表达在哺乳动物细胞中赋予了对RSV的特殊敏感性.
- 针对受体的抗体阻断了RSV (A) 感染在鸟类细胞中.
结论:
- 小型LDLR相关蛋白质作为RSV的细胞受体.
- 这些受体调解RSV的特定进入敏感细胞.
- 了解这些受体对于开发针对特定逆转录病毒的抗病毒战略至关重要.
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