相关实验视频
Updated: May 8, 2026

12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
对P盒子的功能分析,P盒子是林B中的一个域,它是激活Cdc25所需的
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|October 8, 1993
概括
环林B的P盒域对于激活cdc25酸酶至关重要. 这一发现支持细胞循环期间环林B-cdc2复合体激活的两步机制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 环林B-cdc2复合体调节细胞循环的进展.
- 抑制性酸化会维持cdc2的不活化,直到G2晚期.
- 酸酶cdc25激活M阶段进入的环林B-cdc2复合体.
研究的目的:
- 为了研究环林B P盒在cdc25激活中的作用.
- 为了阐明cdc25依赖的环素B-cdc2激活的机制.
主要方法:
- 在cdc2.2的突变环林B蛋白的体外结合试验.
- 细胞循环停止表型的分析.
- 在存在野生型和突变型B环林时,评估cdc25酸酶活性.
主要成果:
- 突变的环林B蛋白与改变的P盒子结合cdc2,但未能激活cdc25.
- 表达突变环林B蛋白的细胞在G2中表现出细胞循环停止.
- cdc25酸酶活性依赖于环林B P盒,对间相和M相形式有明显的调节.
结论:
- 环林B的P盒域对于cdc25激活至关重要.
- 支持用于cdc25依赖的环林B-cdc2复合物的双步激活机制.
- 这项研究阐明了细胞循环控制中的关键监管步骤.
相关概念视频
Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

