通过其3'未翻译区域对tra-2的翻译调节控制了C. elegans的性认同
E B Goodwin1, P G Okkema, T C Evans
1Laboratory of Molecular Biology, University of Wisconsin-Madison 53706.
Cell
|October 22, 1993
概括
在tra-2基因中,直接重复元件 (DREs) 控制其翻译,确保C. elegans发展为雌雄性. 破坏DREs会导致无精子的雌雄性动物,突出显示转化调节.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 线虫C. elegans表现出一种雌性生殖系统,首先产生精子,其次是卵子.
- 在C. elegans中,性别决定对其生命周期至关重要,像tra-2这样的基因发挥着关键作用.
研究的目的:
- 研究位于tra-2基因3'未翻译区域的直接重复元件 (DREs) 的功能.
- 阐明DREs在翻译层面调节tra-2基因表达的机制.
主要方法:
- 在野生型和突变菌株中分析与tra-2信使RNA (mRNA) 的多体组关联.
- 在体外翻译试验中,使用含有DREs的记者RNA进行分析.
- 在DRE中断后评估tra-2mRNA水平.
主要成果:
- 在tra-2 (tra-2(gf)) 中的功能获取突变导致无精子的雌雄性,将它们转化为女性.
- 发现DREs抑制tra-2基因翻译,而不是影响mRNA稳定性或水平.
- 一个RNA结合因子与DREs特别相关,这表明直接的调节相互作用.
结论:
- 通过DREs对tra-2的转化抑制对于C. elegans作为雌雄性/雄性物种的适当发展至关重要.
- 一个RNA结合因子与DREs的相互作用介导了tra-2的转化控制.
- 这项研究揭示了一种对性别决定和发育至关重要的转化调节的新机制.
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