相关实验视频
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Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
一种核孔复合蛋白质,含有指纹图案,结合DNA,并面对核质
1Howard Hughes Medical Institute, Rockefeller University, Laboratory of Cell Biology, New York, New York 10021.
Cell
|January 15, 1993
概括
研究人员发现了一种新的大鼠肝蛋白,nup153,它含有独特的指域. 这种蛋白质与DNA结合,位于核孔综合体,表明它在基因组组织和基因关口中的作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 核素是核孔复合体 (NPC) 的重要组成部分.
- 特定的核波林在核运输和基因组组织中发挥作用.
- 核波林的结构和功能多样性正在不断地被探索.
研究的目的:
- 在大鼠肝脏中识别和表征新型核波林.
- 为了研究已识别的核的功能性质, nup153.3.
- 确定 nup153 在核孔综合体内的定位.
主要方法:
- 鼠肝cDNA的分子克隆和测序.
- 在大肠杆菌中表达指域的表达.
- DNA 结合测定.DNA 结合测定.
- 免疫电子显微镜用于蛋白质定位.
主要成果:
- 克隆和测序了一种鼠肝核素 nup153 (152.8 kd) 的cDNA.
- nup153具有Cys2-Cys2型指纹的新的4倍重复.
- nup153的指域证明了依赖的DNA结合.
- 免疫电子显微镜局部化nup153到NPC的核等离子体侧.
结论:
- nup153是一种新型核蛋白,其指动图介导着DNA结合能力.
- nup153的局部化表明它在NPC组织基因组和封锁可转录基因中的作用.
- nup153可能在基因表达的空间调节中发挥关键作用.
相关概念视频
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Genomic DNA in Eukaryotes
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
DNA Packaging
Overview
DNA Packaging
Overview
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Nucleoid
The nucleoid represents a structurally and functionally distinct region within prokaryotic cells, where the cell's DNA and associated proteins are housed. Unlike eukaryotic cells, prokaryotes lack a membrane-bound nucleus, and the nucleoid facilitates the organization and accessibility of the genetic material within this constraint. The DNA in most bacteria and archaea exists as a single, circular, double-stranded molecule that is highly compacted through supercoiling and interactions with...

