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马德:马克斯的一个异体对象,它对抗了迈克斯的转录活动
D E Ayer1, L Kretzner, R N Eisenman
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cell
|January 29, 1993
概括
研究人员发现了一种新的蛋白质,Mad,与Max合作. 这种Mad-Max复合体,就像Myc-Max一样,结合了DNA,但具有相反的功能,揭示了Max.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 蛋白相互作用 蛋白相互作用
背景情况:
- Myc 蛋白质是转录因子,它们与 Max 异构.
- 马克斯在调节其他调节蛋白的功能中的作用尚未完全理解.
研究的目的:
- 为了确定与Max相互作用的新型蛋白质.
- 描述Max相互作用蛋白的功能和DNA结合特性.
主要方法:
- 使用放射性标记的Max蛋白来选一个lambda gt11表达式库.
- 在体外结合测试以评估蛋白质-蛋白质相互作用.
- 在体内进行交换活化测试以评估转录活性.
主要成果:
- 鉴定了一种新的bHLH-Zip蛋白,Mad,它与Max结合.
- 形成具有类似于Myc-Max的DNA结合特性的Mad-Max异构体.
- Myc-Max和Mad-Max复合体都比Max同极体更受青,并且不受CKII酸化的影响.
- 麦德-马克斯和迈克斯复合体在转录中表现出相反的功能.
结论:
- 马克斯是转录因子网络中的一个中心调解者,涉及Myc和Mad.
- 疯狂代表了马克斯的新功能伙伴,与对立的转录角色对抗Myc.
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