在H子单元费里的生物矿化过程中形成Fe(III) - 铁酸盐复合体
G S Waldo1, J Ling, J Sanders-Loehr
1Department of Biochemistry, North Carolina State University, Raleigh 27695.
概括
研究人员使用光谱检测在费里中发现了一种铁三酸铁复合物. 这种由H子单元形成的中间体,可能解释了与L型相比,H型费里丁的铁生物矿化速度更快.
科学领域:
- 生物化学 生物化学
- 生物矿物化 生物矿物化
- 蛋白质与铁的相互作用
背景情况:
- 费里是一种储存铁的蛋白质.
- 具体的氨基酸侧链协调铁在ferritin之前是未知的.
- 费里丁存在于不同的类型,包括H型和L型,具有不同的铁生物矿化率.
研究的目的:
- 为了确定特定的氨基酸侧链,参与铁中的铁协调.
- 描述在费里矿化过程中形成的中间铁复合物.
- 研究铁酸复合体在H型和L型费里的不同铁生物矿化速率中的作用.
主要方法:
- 在大肠杆菌 (Escherichia coli) 中过度表达牛红细胞铁素.
- 使用紫外线可见光谱 (UV-Vis) 识别铁复合物.
- 使用共振拉曼光谱学对铁复合物的表征.
主要成果:
- 一个铁(III) - 铁酸盐复合物被确定为铁酸铁吸收的早期中间体.
- 这种紫色铁 (III) - 铁酸盐中间体被珀色多核铁 (III) - 氧合物复合物替换为完全矿化费里.
- 只有费里的H亚单位形成了可检测量的铁酸铁酸复合物.
结论:
- 铁(III) - 铁酸盐复合物是铁酸铁矿化中的一个关键中间体.
- 这种复杂物由H子单元的形成可能解释了在H型费里中观察到的更快的铁生物矿化速率.
- 这些发现为铁素中铁的储存和生物矿物化的机制提供了新的见解.
更多相关视频
10:01Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
Published on: November 28, 2017
20.4K
08:55Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
Published on: October 28, 2016
10.9K
相关概念视频
Formation of Complex Ions
26.1K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.1K
Electron Transport Chain: Complex III and IV
9.3K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
9.3K
Receptor Tyrosine Kinases
18.6K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
18.6K
Protein Complex Assembly
16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complexes with Interchangeable Parts
2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Crystal Field Theory - Octahedral Complexes
30.8K
Crystal Field Theory
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
30.8K
