在U1 snRNA中的突变绕过了细胞类型特定RNA剪接因子的要求
K Nandabalan1, L Price, G S Roeder
1Department of Biology, Yale University, New Haven, Connecticut 06511-8112.
Cell
|April 23, 1993
概括
在酵母中,MER1蛋白对MER2基因拼接至关重要. 在U1 snRNA中发生的突变通过改变MER2内部的基配对来绕过这一要求,突出显示了一种新的拼接机制.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 在RNA分离过程中.
背景情况:
- 酵母MER2基因的高效RNA拼接取决于MER1蛋白质,该蛋白质在半分裂过程中得到表达.
- 在MER2拼接中MER1的确切作用以及控制这一过程的机制仍然不完全理解.
研究的目的:
- 为了确定可以绕过MER1在MER2RNA拼接中的MER1要求的遗传因素.
- 阐明了MER1独立的MER2拼接背后的分子机制.
主要方法:
- 采用遗传选择策略来隔离抑制MER1依赖的第二位基因突变.
- 进行了抑制器突变的序列分析和RNA-DNA相互作用的分析.
主要成果:
- 在SNR19中发生的一种突变,编码U1小核RNA (snRNA),被确定为抑制剂.
- 这种突变改变了U1 snRNA序列,使其能够与MER2内突的某个位点与正规的5'拼接位点以外的位点结合.
- 在MER2 5'拼接部位恢复共识序列也减轻了MER1的要求,与U1 snRNA基配对增加相关.
结论:
- 通过影响U1 snRNA相互作用的突变,可以绕过MER1蛋白对MER2拼接的要求.
- 这表明U1 snRNA可以与内子内的非正规位点接触,以在某些条件下促进拼接.
- 这些发现为结合部位识别的灵活性和酵母中介质基因结合的调节提供了新的见解.
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