FAR1将信号传导通路与酵母菌的细胞循环机制联系起来
M Peter1, A Gartner, J Horecka
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Cell
|May 21, 1993
概括
阿尔法因子通过FUS3激酶和FAR1蛋白触发酵母细胞循环停止. 通过FUS3对FAR1的酸化调节了它与CDC28-CLN2的相互作用,将信号与细胞周期控制联系起来.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 酵母 (Saccharomyces cerevisiae) 中的α因子费洛蒙会在G1阶段阻止细胞的发生.
- 这种逮捕对于交配至关重要,并且涉及抑制G1环林.
- FUS3 (MAP激酶) 和FAR1都与这种细胞循环停止机制有关.
研究的目的:
- 阐明FAR1对G1细胞循环停止的分子机制.
- 为了研究FUS3,FAR1和CDC28-CLN2循环素依赖激酶复合体之间的关系.
主要方法:
- 在体外激酶试验测试以确定FAR1.1的FUS3酸化.
- 对FAR1-CDC28-CLN2复合体形成的分析.
- 在体内酸化的研究.
- 对FAR1.1的突变分析.
主要成果:
- FAR1 是 FUS3 激酶的直接基质.
- 通过FUS3介导的FAR1酸化调节了它与CDC28-CLN2激酶的关联.
- FAR1也被CDC28-CLN2以细胞周期依赖的方式酸化.
- FAR1结合CDC28-CLN2的能力与其细胞循环停止功能相关.
结论:
- 费洛蒙信号通路与细胞核心循环机制之间的关键环节是FAR1.
- 涉及FUS3和CDC28-CLN2的酸化事件是FAR1功能中的关键调节步骤.
- 这些发现为交配费洛蒙如何控制酵母细胞周期提供了分子基础.
相关概念视频
Yeast Signaling
18.5K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
18.5K
MAPK Signaling Cascades
9.2K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.2K
Cells Coordinate Growth and Proliferation
5.3K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.3K
Mitogens and the Cell Cycle
8.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.4K
The Cell Cycle Control System
6.4K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
6.4K
The Cell Cycle Control System
14.9K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
14.9K


