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在老鼠中,端粒特异性染色质的核体组织
V L Makarov1, S Lejnine, J Bedoyan
1Department of Biological Sciences, University of Michigan, Ann Arbor 48109-2099.
Cell
|May 21, 1993
概括
哺乳动物的端粒,染色体的保护帽,结构类似于核细胞,类似于大量的染色体. 这与较低的真核生物形成鲜明对比,其中端粒缺乏这种核体组织.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 端粒是真核染色体末端的关键保护性结构.
- 哺乳动物端粒的结构组织,特别是与染色素的关系,仍然是一个研究领域.
研究的目的:
- 为了研究大鼠肝脏的核体结构,分相染色体端粒.
- 为了比较端粒染色质结构与较低的真核生物的大量染色质和端粒.
主要方法:
- 微球菌核酶消化孤立的核.
- 由此产生的颗粒的糖分梯度沉积分析.
- 核蛋白和DNA凝电泳. 核蛋白和DNA凝电泳.
- DNAase I 的消化和分析.
主要成果:
- 鼠肝端粒长度为20-100 kb,在微球菌核酶消化时表现出157 bp的周期性,形成类似于寡核细胞体的粒子.
- 单体端粒颗粒与核细胞核颗粒共迁移,但不结合基因组H1.1.
- DNAase I 消化揭示了端粒核蛋白中的 ~10.4 bp 间距,反映了大量的核细胞.
- 移除组素H1影响端粒沉积类似于散装染色体.
结论:
- 哺乳动物的端粒被组织成距离很近的核体,类似于大量的染色体.
- 端粒的核体组织不同于在较低的真核生物中观察到的缺乏这种结构.
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