在花马赛克病毒35SRNA上非线性核糖体迁移
J Fütterer1, Z Kiss-László, T Hohn
1Friedrich Miescher-Institute, Basel, Switzerland.
Cell
|May 21, 1993
概括
花草马赛克病毒35SRNA领导序列可以抑制翻译,但特定元素允许植物细胞下游基因表达. 这一过程涉及通过扫描复合体绕过内部区域,甚至在单独的RNA分子之间.
科学领域:
- 分子生物学分子生物学
- 植物病毒学 植物病毒学
- 基因表达规范 基因表达规范
背景情况:
- 花草马赛克病毒 (CaMV) 35SRNA具有600个核酸领导序列.
- 这个领导者包含小的开放阅读框架 (sORFs),可以抑制下游编码区域的翻译.
研究的目的:
- 调查CaMV 35S RNA领导者内的下游编码区域的翻译管理的监管机制.
- 识别特定的RNA元素对于某些植物细胞的翻译启动至关重要或不可或缺.
主要方法:
- 对CaMV 35SRNA领导序列及其对下游翻译的影响的分析.
- 领导序列的突变发生,包括插入干环结构和编码区域.
- 研究特定植物细胞类型的翻译.
- 检查单独的RNA分子之间的跨作用变换.
主要成果:
- 允许下游编码区域的翻译在特定的植物细胞中,这取决于RNA 5'终端和其他领导元素.
- 领导者的中央部分是可用的,或者可以容纳插入,而不取消翻译.
- 一种称为"shunting"的现象允许扫描复合体绕过抑制区域.
- 此外,还证明了在不同RNA分子之间发生的转移中发生了变换.
结论:
- 该CaMV 35S RNA领导者采用一个调节机制来克服sORFs的翻译抑制.
- 这种变换允许下游基因的高效表达,由特定的RNA特征调节.
- 轮回能力是一种灵活的机制,可以同时运行cis和trans.
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