来自菌根真菌Glomus versiforme的酸盐运输体
1Samuel Roberts Noble Foundation, Plant Biology Division, Ardmore, Oklahoma 73401, USA.
Nature
|December 7, 1995
概括
膀状-状 (VA) 菌根真菌可以增强植物的营养吸收. 研究人员在Glomus versiforme中发现了一种新的酸盐转运体 (GvPT),对这种共生过程至关重要.
科学领域:
- 植物和真菌的共生是植物的共生.
- 分子生物学分子生物学
- 营养物质的运输 营养物质的运输
背景情况:
- 囊状-状 (VA) 菌根真菌与大多数陆地植物形成关键的共生关系.
- 这些真菌有助于植物吸收矿物质营养物质,特别是酸盐,对于生长至关重要,特别是在营养限制条件下.
- 由于VA真菌的义务共生性质,对真菌酸盐吸收的分子机制的理解是有限的.
研究的目的:
- 调查在VA菌根协会中由真菌介导的酸盐吸收的分子机制.
- 为了识别和描述VA菌根真菌Glomus versiforme的酸盐输送体.
主要方法:
- 鉴定了一种辅助DNA (cDNA),编码来自Glomus versiforme的跨膜酸盐输送体 (GvPT).
- 通过补充酵母酸盐运输突变物来确认GvPT的功能.
- 在菌根结合期间,G. versiforme中GvPT表达的定位.
主要成果:
- 从Glomus versiforme.发现了一个编码跨膜酸盐载体的辅助DNA (cDNA),名为GvPT.
- 证实GvPT蛋白质通过酵母突变的补充作用作为酸盐载体.
- 发现GvPT的表达在G. versiforme的外部叶中,这是土壤酸盐吸收的主要部位.
结论:
- 这项研究确定了VA菌根真菌Glomus versiforme中的一个关键的跨膜酸盐输送体 (GvPT).
- GvPT在真菌最初从土壤中吸收酸盐方面发挥着重要作用.
- 这一发现为植物-真菌共生中有益的营养交换提供了分子洞察力.
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