通过PHO80-PHO85环林-CDK复合物的PHO4核定位的调节
E M O'Neill1, A Kaffman, E R Jolly
1Department of Biochemistry and Biophysics, University of California at San Francisco, School of Medicine 94143-0448, USA.
Science (New York, N.Y.)
|January 12, 1996
概括
由PHO80-PHO85环林-CDK复合体对PHO4转录因子的酸化调节了PHO5基因表达. 这个过程控制着PHO4的控制.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 基因规则 基因规则
背景情况:
- 酵母中的PHO5基因是在酸盐饥饿期间诱导的.
- PHO4是一种转录因子,对PHO5基因激活至关重要.
- PHO80-PHO85复合体是一种依赖于环林的激酶,参与调节基因表达.
研究的目的:
- 研究PHO4酸化在调节PHO5基因转录中的作用.
- 确定PHO80-PHO85复合体如何影响PHO4的局部化和功能.
- 确定PHO4酸化的特定部位及其对基因抑制的影响.
主要方法:
- 使用了酵母遗传学和分子生物学技术.
- 在不同的酸盐条件下分析PHO4蛋白位址.
- 使用生物化学方法识别PHO4酸化位点.
主要成果:
- 在富含酸盐的条件下,PHO4被PHO80-PHO85复合物酸化.
- 酸化导致PHO4在细胞质中积累,抑制PHO5的转录.
- 在酸盐饥饿期间,PHO4定位到核中,激活PHO5转录.
- 在PHO4上确定了特定的酸化点,并证实它们对于抑制至关重要.
结论:
- 由PHO80-PHO85对PHO4的酸化是抑制PHO5转录的关键机制.
- PHO80-PHO85复合体通过控制PHO4的核进口来调节PHO5的表达.
- 这项研究揭示了酵母菌中酸盐稳态的关键调节途径.
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