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在运动神经元生成中对LIM homeobox基因Isl1的要求揭示了内部神经元分化中的运动神经元依赖的步骤

S L Pfaff¹, M Mendelsohn, C L Stewart

¹Howard Hughes Medical Institute, Department of Biochemistry and Molecule Biophysics, Columbia University, New York, New York 10032, USA.

|January 26, 1996

概括

岛屿1 (ISL1) 对于运动神经元生成至关重要. 没有ISL1,运动神经元和一些内部神经元无法发育,表明ISL1.

科学领域:

发育生物学是发展生物学.
神经科学是一个神经科学.
遗传学是一个遗传学.

背景情况:

运动神经元的分化涉及到LIM家庭主体转录因子Islet1 (ISL1).
了解ISL1的作用对于理解神经发育至关重要.

研究的目的:

研究ISL1对于运动神经元生成的必要性.
分析ISL1缺失对神经管细胞分化的影响.

主要方法:

基因向消除ISL1在胚胎神经管中的表达.
在ISL1缺陷胚胎中的细胞分化分析.

主要成果:

ISL1对于运动神经元产生是不可或缺的;没有它就不会产生神经元.
虽然大多数神经管分化正常进行,但表达Engrailed1 (EN1) 的内部神经元也无法分化.
通过与含有运动神经元的神经组织共同培养,可以挽救EN1+内部神经元的分化.

结论:

ISL1是运动神经元发育所需的转录因子.
运动神经元的产生似乎是区分特定内部神经元群体的先决条件.

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