E-cadherin是内在素的受体,内在素是L. monocytogenes进入上皮细胞所需的表面蛋白质
J Mengaud1, H Ohayon, P Gounon
1Unité des Interactions Bactéries-Cellules, Station Centrale de Microscopie Electronique, Institut Pasteur, Paris, France.
Cell
|March 22, 1996
概括
研究人员将E-cadherin确定为内内素的细胞受体,内内素是一种Listeria monocytogenes蛋白质. 这种相互作用调解了细菌进入非细胞的过程,揭示了宿主-病原体相互作用的新机制.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 格拉姆阳性细菌,如Listeria monocytogenes,可以入侵宿主细胞.
- 特定的细胞受体和媒介细菌进入非细胞细胞的机制尚未完全理解.
- 内部素是L. monocytogenes的关键毒性因子,对其入侵至关重要.
研究的目的:
- 为了识别内部素的细胞受体,这是一种对L. monocytogenes进入宿主细胞至关重要的蛋白质.
- 阐明L. monocytogenes进入非细胞细胞的机制.
主要方法:
- afinity 染色法被用来识别内部alin. 的联结体.
- 功能性测试包括用肉E-cadherin同类物 (L-CAM) 传染纤维细胞,并测量细菌进入.
- 进行了抗体阻断实验以确认受体特异性.
主要成果:
- 鉴定出E-cadherin是细胞内毒素的细胞受体.
- 在纤维细胞中L-CAM的表达显著增强了L. monocytogenes的输入.
- 此外,L-CAM表达还促进了表达内部蛋白的重组L. innocua菌株的进入.
- 针对L-CAM的抗体阻断了内部中介细菌的进入.
- 李斯特菌的进入是通过诱导的细胞形成而发生的,没有膜,与沙门氏菌的进入不同.
结论:
- 埃卡德林是细胞受体,通过内部蛋白质调解L. monocytogenes通过非细胞进入非细胞.
- 这种相互作用代表了一种新的细菌入侵机制和一种新型的E-cadherin异性恋相互作用.
- 了解这种途径可以了解宿主-病原体相互作用和潜在的治疗点.
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