番茄Cf-2疾病耐药部位包括两个编码富含白蛋白重复蛋白的功能基因
M S Dixon1, D A Jones, J S Keddie
1Sainsbury Laboratory, John Innes Centre, Norwich, United Kingdom.
Cell
|February 9, 1996
概括
植物抵抗基因,如Cf-2,对于病原体防御至关重要. 这项研究分离了番茄Cf-2基因,揭示了它们在识别来自C. fulvum的特定激烈性 (Avr) 基因中的作用,增强了植物免疫力.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病原体相互作用
- 遗传学 是一个遗传学.
背景情况:
- 植物疾病耐药性往往是由主导性耐药性基因调解的.
- 假设这些基因的产物可以检测病原体编码的激发性 (Avr) 蛋白质.
- 番茄叶菌病原体*C. fulvum*作为研究植物病原体识别的模型.
研究的目的:
- 为了隔离和表征番茄耐药部位Cf-2.
- 了解Cf-2-介导抗 *C. fulvum* 的分子基础.
- 研究Cf-2蛋白质的结构特征及其在植物防御信号传递中的潜在作用.
主要方法:
- 位置克隆被用来从番茄中分离Cf-2位点.
- 对已识别的Cf-2基因进行了序列分析.
- 与其他已知的耐药性基因进行了比较分析,例如Cf-9.
主要成果:
- Cf-2位点包含两个非常相似的基因,每个基因都对表达相应的Avr2基因的特定*C. fulvum*菌株产生抗性.
- Cf-2基因编码的蛋白质有38个丰富于白的重复 (LRR) 基因,其中20个具有保存的交替重复.
- Cf-2蛋白的C终端区域与Cf-9基因产物具有同质性.
结论:
- 已识别的Cf-2基因对于识别*C. fulvum*恶毒因子至关重要.
- Cf-2蛋白质的结构特征,特别是LRR动机,可能与病原体识别有关.
- 保存的C端区域可能与下游信号组件相互作用,以激活植物防御机制.
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