一个GroEL-GroES介导的蛋白质折叠反应的活性中间体的表征
J S Weissman1, H S Rye, W A Fenton
1Department of Genetics, Yale School of Medicine, New Haven, Connecticut 06510, USA.
Cell
|February 9, 1996
概括
蛋白质折叠可以发生在GroEL/GroES chaperonin复合体内. 在GroES释放之前,基质在GroEL通道内完成折叠,这表明cis三元复合物是活性折叠中间体.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 蛋白质折叠 蛋白质的折叠
背景情况:
- 像GroE这样的伴侣蛋白促进蛋白质折叠.
- 之前的模型建议在折叠完成之前释放基质.
研究的目的:
- 为了调查蛋白质折叠是否发生在GroEL通道内.
- 确定 cis 三元复合体在 GroE 中介折叠中的作用.
主要方法:
- 使用pyrene-rhodanese-GroEL复合物的停止流光异质.
- 利用不可水解的ATP类似物和GroEL突变物来阻止GroES的释放.
主要成果:
- 在GroEL中,GroES和ATP的添加诱导了基板灵活性的快速变化.
- 当GroES释放被抑制时,基质在与Chaperonin结合时完成折叠.
- 折叠启动发生在三元复合体中.
结论:
- 与ATP一起的cis三元复合体是GroeE介导的蛋白质折叠中的活性中间体.
- 在GroES释放之前,可以在GroEL通道内完成蛋白质折叠.
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