在C. elegans vulval诱导过程中,细胞连接蛋白LIN-7对LET-23受体进行定位
J S Simske1, S M Kaech, S A Harp
1Department of Developmental Biology, Stanford University Medical School, California 94305-5427, USA.
Cell
|April 19, 1996
概括
在C. elegans中,LIN-7蛋白将LET-23受体氨酸激酶定位到细胞结点,这对阴道发育至关重要. LIN-2和LIN-7对于这种受体定位和信号活动至关重要.
科学领域:
- 发育生物学 发展生物学
- 细胞信号传递 细胞信号传递
- 分子遗传学 分子遗传学
背景情况:
- 在C. elegans中, Vulva的形成是由细胞信号发送所启动的.
- 这一过程涉及一个受体氨酸激酶 (RTK) 保护通路.
- 在lin-2和lin-7中发生的突变会破坏阴道发育,模仿RTK通路缺陷.
研究的目的:
- 研究LIN-2和LIN-7影响阴道发育的分子机制.
- 为了确定LIN-7之间的关系,LET-23RTK,和细胞结点定位.
- 了解LET-23受体局部化对于正确信号传输的要求.
主要方法:
- 在C. elegans中对lin-2和lin-7突变的遗传分析.
- 免疫光显微镜以确定蛋白质定位.
- 对LET-23受体氨酸激酶局部化和功能的分析.
- 过度表达研究以评估救援表型.
主要成果:
- 鉴定出LIN-7是一种细胞结关联蛋白,直接与LET-23RTK结合.
- LET-23 RTK局部化到细胞结点,LIN-2和LIN-7都对这种局部化有必要.
- LET-23的过度表达在lin-2和lin-7突变体中挽救了无阴道的表型,表明对错位化进行补偿.
结论:
- 适当地将LET-23受体氨酸激酶定位到Pn.p细胞结点对于阴道发育信号提供至关重要.
- LIN-2和LIN-7在细胞结点调节LET-23局部化方面发挥着至关重要的作用.
- 受体密度可以部分补偿受体局部化的缺陷.
相关概念视频
Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
Mechanism of Lamellipodia Formation
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
Anchoring Junctions
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
Adherens Junctions
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Adherens Junctions are Dynamic
The endothelial cells...


