神经生长因子是记忆B淋巴细胞的自身隐性生存因子
M Torcia1, L Bracci-Laudiero, M Lucibello
1Department of Experimental Medicine, University of Rome "Tor Vergata", Italy.
Cell
|May 3, 1996
概括
神经生长因子 (NGF) 作为记忆B淋巴细胞的自身隐性生存因子. 阻断NGF会导致记忆B细胞的死亡,并减少体内抗体的产生.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 神经科学是一个神经科学.
背景情况:
- 神经生长因子 (NGF) 对神经元发育和生存至关重要.
- 在淋巴细胞功能中NGF的作用,特别是B细胞存活率,尚未完全理解.
研究的目的:
- 研究NGF在人体淋巴细胞中的产生和功能.
- 确定NGF是否起到B淋巴细胞,特别是记忆B细胞的生存因子的作用.
主要方法:
- 培养人类T和B淋巴细胞和巨细胞以评估NGF生产.
- 利用对NGF的中和抗体研究其对淋巴细胞存活和功能的影响.
- 在淋巴细胞中测量Bcl-2蛋白水平和细胞死亡.
- 在体内对用特定抗原免疫的小鼠进行实验,以评估抗NGF抗体对抗体标位和B细胞群的影响.
主要成果:
- B细胞构成性地产生NGF并表达其受体 (p140trk-A和p75NGFR).
- 内源NGF的中和诱导了B型记忆淋巴细胞 (IgG+或IgA+) 的亡,但不发生在B型原始淋巴细胞 (IgM+/IgD+) 中.
- 在体内给予抗NGF抗体,可降低免疫小鼠的特定IgG抗体标位和IgG+或IgA+B细胞数量.
结论:
- NGF 是一种特定于记忆B淋巴细胞的自身隐性生存因子.
- NGF在维持产生抗体的B细胞的生存和调节幽默免疫力方面发挥着至关重要的作用.
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