复合叶的制造:在番茄中对叶子结构的基因操纵
D Hareven1, T Gutfinger, A Parnis
1Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
Cell
|March 8, 1996
概括
该研究显示,玉米的Notted-1 (Kn1) 基因可以诱导复合叶中的众多叶片,但只会导致单叶的形,这表明叶形的不同发育程序.
科学领域:
- 植物生物学 植物生物学
- 发育遗传学的发展遗传学.
- 形态发生 形态发生 形态发生
背景情况:
- 叶子的复杂性,无论是简单的还是复合的,都是植物的一个关键形态特征.
- 了解叶子发育的遗传基础对于植物科学至关重要.
研究的目的:
- 为了研究基因机制的基础上,简单和复合叶之间的区别.
- 确定玉米Notted-1 (Kn1) 基因在叶子发育中的作用及其与叶子复杂性的相互作用.
主要方法:
- 利用番茄 (Solanum lycopersicum) 中的玉米 Knotted-1 (Kn1) 基因,其中具有影响叶子复合性的突变.
- 分析Kn1错误表达对复合叶植物和单叶植物 (西红和阿拉比多普西斯萨利亚纳) 叶形态的影响.
- 检查了番茄 Kn1 ortholog 在叶原始的表达模式.
主要成果:
- 番茄中Kn1的错误表达诱导了多达2000个叶片的形成,在复合叶中以重复单位组织.
- 相比之下,单片叶 (Arabidopsis或单片叶番茄突变种) 中的Kn1误表导致形,但没有叶子分枝.
- 番茄 Kn1 ortholog 显示表达在叶子中,与其 Arabidopsis 对应物不同.
结论:
- 主叶附属体的发育程序决定了对Kn1误表的反应,导致简单叶与复合叶的结果不同.
- 这表明,不同的遗传路径是简单和复合叶架构的基础.
- 叶子形式二分法很可能在叶子早期发育过程中通过差异性基因调节来控制.
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