TAFII250是一种双分蛋白激酶,可酸化基基转录因子RAP74
R Dikstein1, S Ruppert, R Tjian
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology University of California, Berkeley, 94720, USA.
Cell
|March 8, 1996
概括
TAFII250蛋白作为一种激酶,酸化RAP74,以调节转录. 在TFIID复合体内,TAFII250的这种向酸化表明了基因转录中的新信号机制.
科学领域:
- 分子生物学分子生物学
- 基因转录 基因转录
- 蛋白质激酶是一种蛋白质激酶.
背景情况:
- 转录因子TFIID子单元 (TAFs) 调解蛋白相互作用和转录的DNA识别.
- 对于TFIID功能的精确监管机制的理解仍然不完全.
研究的目的:
- 为了研究纯化重组TAFII250.0.的酶活性.
- 为了确定TAFII250是否酸化其他转录因子,特别是RAP74.
主要方法:
- 再组合TAFII250.0.的净化方法
- 采用TAFII250和各种基质,包括RAP74和其他基底转录因子的体外激酶试验.
- 删除TAFII250的分析,以确定功能性激酶域.
- 酸化位点分析 (血清残留物).
主要成果:
- 纯化重组TAFII250表现出蛋白质血清激酶活性.
- TAFII250可选择性化RAP74,但不能对其他测试的蛋白质进行化.
- 通过TAFII250对RAP74的酸化发生在完整的TFIID复合体内.
- TAFII250 具有两种激酶域,这两种都需要在血清残留物上进行高效的 RAP74 转.
结论:
- TAFII250作为一种酶,可以向RAP74进行酸化.
- 这种在TFIID复合体内发生的酸化事件表明TAFII250在转录启动中起着调节作用.
- 这些发现提出了一种信号通路,涉及TAFII250介导的RAP74酸化来调节转录.
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